All my previous research has been with this construct. I have injected embryos with the unmodified construct, which revealed that the construct has all the elements necessary to drive the expression of the orange fluorescent protein. That's all my research from the C.A.S. program.
I then dived into working in more of an "abstract" way with the construct. I analyzed the NvFoxB gene (in this picture, it's the teal color) in two sequencing databases, Jaspar and Possum. I was searching for possible TATA binding sites encoded within this gene. The programs gave me three places that had a high probably of being a TATA binding site (these are in dark blue).
I then took all this data and began using ApE, a sequence analyzing program. I searched for restriction enzymes that would cut the construct in specific places, separating these possible TATA binding sites (the dark purple colors are the restriction enzymes I chose).
That's pretty much what this picture is showing. From this, I now have to build my own constructs, which is turning into a crazy fiasco as all my experiments have just epically failed, mostly because I'm a goofball and end up messing up really simple things. Such as autoclaving petri dishes... who does that? Me. We have a little corner where we're putting all the "this is what you DON'T DO" examples, and all of them are from me so far... pretty embarrassing!
